Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells.
Identifieur interne : 002B80 ( Main/Exploration ); précédent : 002B79; suivant : 002B81Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells.
Auteurs : Lanying Du [États-Unis] ; Guangyu Zhao ; Lin Li ; Yuxian He ; Yusen Zhou ; Bo-Jian Zheng ; Shibo JiangSource :
- Biochemical and biophysical research communications [ 1090-2104 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (biosynthèse), Cellules CHO, Cricetinae, Cricetulus, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (immunologie), Interleukine-4 (immunologie), Lymphocytes T (immunologie), Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (immunologie), Récepteurs viraux (immunologie), Souris, Souris de lignée BALB C, Structure tertiaire des protéines, Syndrome respiratoire aigu sévère (), Vaccination, Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Anticorps antiviraux, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Glycoprotéines membranaires, Interleukine-4, Lymphocytes T, Protéines de l'enveloppe virale, Récepteurs viraux, Virus du SRAS.
- Animaux, Cellules CHO, Cricetinae, Cricetulus, Femelle, Glycoprotéine de spicule des coronavirus, Souris, Souris de lignée BALB C, Structure tertiaire des protéines, Syndrome respiratoire aigu sévère, Vaccination.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (biosynthesis), CHO Cells, Cricetinae, Cricetulus, Female, Interleukin-4 (immunology), Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Protein Structure, Tertiary, Receptors, Virus (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, T-Lymphocytes (immunology), Vaccination, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Interleukin-4, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- immunology : SARS Virus, T-Lymphocytes.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, CHO Cells, Cricetinae, Cricetulus, Female, Mice, Mice, Inbred BALB C, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Vaccination.
Abstract
The receptor-binding domain (RBD) of SARS coronavirus (SARS-CoV) spike (S) protein contains multiple conformation-dependent epitopes that induce neutralizing antibody responses. Here we used CHO-K1 cells to establish a cell line for stable expression of a 193-mer (residues 318-510) RBD (RBD193-CHO) and determined its antigenicity and immunogenicity. We found that RBD193-CHO reacted strongly with a panel of six monoclonal antibodies recognizing various conformational and linear epitopes in RBD, suggesting that this recombinant protein maintains intact conformation and good antigenicity. Immunization of mice with RBD193-CHO resulted in induction of high titers of RBD-specific neutralizing antibodies and potent IL-4-expressing T cell responses. RBD193-CHO induced immunity that protected a majority of the vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD produced in an established stable cell line maintains strong immunogenicity with high potential for use as an effective and economic subunit SARS vaccine.
DOI: 10.1016/j.bbrc.2009.05.003
PubMed: 19422787
Affiliations:
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Le document en format XML
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Kimball Research Institute, New York Blood Center, New York, NY 10065, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY 10065</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name></author><author><name sortKey="Li, Lin" sort="Li, Lin" uniqKey="Li L" first="Lin" last="Li">Lin Li</name></author><author><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="eISSN">1090-2104</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>CHO Cells</term><term>Cricetinae</term><term>Cricetulus</term><term>Female</term><term>Interleukin-4 (immunology)</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Protein Structure, Tertiary</term><term>Receptors, Virus (immunology)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Spike Glycoprotein, Coronavirus</term><term>T-Lymphocytes (immunology)</term><term>Vaccination</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Cellules CHO</term><term>Cricetinae</term><term>Cricetulus</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Interleukine-4 (immunologie)</term><term>Lymphocytes T (immunologie)</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Récepteurs viraux (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Structure tertiaire des protéines</term><term>Syndrome respiratoire aigu sévère ()</term><term>Vaccination</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Interleukin-4</term><term>Membrane Glycoproteins</term><term>Receptors, Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Interleukine-4</term><term>Lymphocytes T</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>CHO Cells</term><term>Cricetinae</term><term>Cricetulus</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Protein Structure, Tertiary</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules CHO</term><term>Cricetinae</term><term>Cricetulus</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Structure tertiaire des protéines</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The receptor-binding domain (RBD) of SARS coronavirus (SARS-CoV) spike (S) protein contains multiple conformation-dependent epitopes that induce neutralizing antibody responses. Here we used CHO-K1 cells to establish a cell line for stable expression of a 193-mer (residues 318-510) RBD (RBD193-CHO) and determined its antigenicity and immunogenicity. We found that RBD193-CHO reacted strongly with a panel of six monoclonal antibodies recognizing various conformational and linear epitopes in RBD, suggesting that this recombinant protein maintains intact conformation and good antigenicity. Immunization of mice with RBD193-CHO resulted in induction of high titers of RBD-specific neutralizing antibodies and potent IL-4-expressing T cell responses. RBD193-CHO induced immunity that protected a majority of the vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD produced in an established stable cell line maintains strong immunogenicity with high potential for use as an effective and economic subunit SARS vaccine.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Li, Lin" sort="Li, Lin" uniqKey="Li L" first="Lin" last="Li">Lin Li</name><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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